The road from qualitative to quantitative assay: What is next?
نویسنده
چکیده
INTRODUCTION 110 PRE-PCR STEPS 111 INVENTIONS MADE IN “ABSOLUTE” QUANTIFICATION ASSAYS 112 HOW THE RELATIVE QUANTIFICATION STRATEGY CHANGED 114 WHAT ABOUT PCR EFFICIENCY? 115 ASSAY VARIANCE AND HOW TO PERFORM A PROPER NORMALIZATION 116 EXPRESSION PROFILING, qPCR BIOINFORMATICS, AND STATISTICAL ANALYSIS 117 WHAT IS NEXT IN REAL-TIME PCR? 118 GENE EXPRESSION AND MORE – THE SYSTEM BIOLOGY IDEA 120 CONCLUSION 124 REFERENCES 124
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